Recombinant:Article Title: Thrombin-derived C-terminal peptides bind and form aggregates with sulfated glycosaminoglycans.
Article Snippet: .. 1432-1033.1980.tb05997.x) Chemicals, peptides, and recombinant proteins Chloramphenicol Duchefa Biochemie C01130025 Carbomycin Smolecules S522695 IPTG Swab I1000-5 Tris AppliChem A1086,5000 LB medium Sigma-Aldrich L3022-1 KG Urea Duchefa Biochemie U1363.5000 Imidazole VWR Life Science 0527-100G The thrombin-derived peptide TCP-25 AmbioPharm, Inc. https://www.ambiopharm.com Human α-thrombin Innovative RESEARCH https://www.innov-research.com Human prothrombin Innovative RESEARCH https://www.innov-research.com Human γ-thrombin Innovative RESEARCH https://www.innov-research.com Human neutrophil elastase Sigma-Aldrich 324681 Heparin Sigma-Aldrich H3393–100KU Chondroitin sulfate B Sigma-Aldrich C-3788 10–20 % Novex Tricine pre-cast gel Life Technologies EC6625BOX NativePAGE Bis-Tris Gels System 4–16 % Life Technologies BN2111BX10 Trans-Blot® TurboTM Mini PVDF Transfer Packs Bio-Rad 170–4156 SuperSignal West Pico Chemiluminescent Substrate Thermo Scientific 34080 Thiflavin T Sigma-Aldrich T3516-5G Software and algorithms Image Lab 6.1 Bio-Rad Laboratories, Inc. https://www.bio-rad.com/en-se/product/image-lab-software?ID=KRE6P5E8Z&s_kwcid=AL% 2118120%213%21657783331339%21e%21%21g%21%21image+lab+6.1&WT_mc_ id=240108040415&WT_srch=1&WT_knsh_id=_kenshoo_clickid_&gad_ source=1&gclid=CjwKCAiAlcyuBhBnEiwAOGZ2Sx1wEDhwRKZie6RbuYzgJKZn3w8TNxw_ 5KpD6eLJtSmUDWRTTEcZkxoCRhQQAvD_BwE GraphPad Prism v10.0. .. GraphPad Software https://www.graphpad.com/features Spectra ManagerTM Suite Spectroscopy Software Jasco Inc. https://jascoinc.com/products/spectroscopy/molecular-spectroscopy-software/ MO.Affinity Analysis Software NanoTemper https://shop.nanotempertech.com/en/moaffinity-analysis-software-unlimited-licenses-34 Modeller v9.21 Sali and Blundell (1994) https://salilab.org/modeller/ CHARMM-GUI Solution Builder Jo et al. (2008) https://charmm-gui.org/?doc=input/solution CHARMM-GUI Glycan Reader and Modeller Park et al. (2019) https://charmm-gui.org/?doc=input/glycan GROMACS 2021 Abraham et al. (2015) https://www.gromacs.org VMD 1.9 Humphrey and Dalke (1996) https://www.ks.uiuc.edu/Research/vmd/ Other Carbon-coated grids (copper mesh, 400) TED PELLA, INC. 01754–7 (continued on next page) G. Petruk et al. Heliyon 10 (2024) e35703 (continued ) REAGENT or RESOURCE SOURCE IDENTIFIER Immobilized Metal Affinity Chromatography (IMAC) (His-TrapNickel-chelating columns Invitrogen R901-15
Software:Article Title: Thrombin-derived C-terminal peptides bind and form aggregates with sulfated glycosaminoglycans.
Article Snippet: .. 1432-1033.1980.tb05997.x) Chemicals, peptides, and recombinant proteins Chloramphenicol Duchefa Biochemie C01130025 Carbomycin Smolecules S522695 IPTG Swab I1000-5 Tris AppliChem A1086,5000 LB medium Sigma-Aldrich L3022-1 KG Urea Duchefa Biochemie U1363.5000 Imidazole VWR Life Science 0527-100G The thrombin-derived peptide TCP-25 AmbioPharm, Inc. https://www.ambiopharm.com Human α-thrombin Innovative RESEARCH https://www.innov-research.com Human prothrombin Innovative RESEARCH https://www.innov-research.com Human γ-thrombin Innovative RESEARCH https://www.innov-research.com Human neutrophil elastase Sigma-Aldrich 324681 Heparin Sigma-Aldrich H3393–100KU Chondroitin sulfate B Sigma-Aldrich C-3788 10–20 % Novex Tricine pre-cast gel Life Technologies EC6625BOX NativePAGE Bis-Tris Gels System 4–16 % Life Technologies BN2111BX10 Trans-Blot® TurboTM Mini PVDF Transfer Packs Bio-Rad 170–4156 SuperSignal West Pico Chemiluminescent Substrate Thermo Scientific 34080 Thiflavin T Sigma-Aldrich T3516-5G Software and algorithms Image Lab 6.1 Bio-Rad Laboratories, Inc. https://www.bio-rad.com/en-se/product/image-lab-software?ID=KRE6P5E8Z&s_kwcid=AL% 2118120%213%21657783331339%21e%21%21g%21%21image+lab+6.1&WT_mc_ id=240108040415&WT_srch=1&WT_knsh_id=_kenshoo_clickid_&gad_ source=1&gclid=CjwKCAiAlcyuBhBnEiwAOGZ2Sx1wEDhwRKZie6RbuYzgJKZn3w8TNxw_ 5KpD6eLJtSmUDWRTTEcZkxoCRhQQAvD_BwE GraphPad Prism v10.0. .. GraphPad Software https://www.graphpad.com/features Spectra ManagerTM Suite Spectroscopy Software Jasco Inc. https://jascoinc.com/products/spectroscopy/molecular-spectroscopy-software/ MO.Affinity Analysis Software NanoTemper https://shop.nanotempertech.com/en/moaffinity-analysis-software-unlimited-licenses-34 Modeller v9.21 Sali and Blundell (1994) https://salilab.org/modeller/ CHARMM-GUI Solution Builder Jo et al. (2008) https://charmm-gui.org/?doc=input/solution CHARMM-GUI Glycan Reader and Modeller Park et al. (2019) https://charmm-gui.org/?doc=input/glycan GROMACS 2021 Abraham et al. (2015) https://www.gromacs.org VMD 1.9 Humphrey and Dalke (1996) https://www.ks.uiuc.edu/Research/vmd/ Other Carbon-coated grids (copper mesh, 400) TED PELLA, INC. 01754–7 (continued on next page) G. Petruk et al. Heliyon 10 (2024) e35703 (continued ) REAGENT or RESOURCE SOURCE IDENTIFIER Immobilized Metal Affinity Chromatography (IMAC) (His-TrapNickel-chelating columns Invitrogen R901-15
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